Leica DM6B Fluorescence Microscopy

The Leica DM6 B upright microscope is equipped with Hamamatsu sCMOS camera, Leica DFC450 color CCD, Spectra X LED light engine, and LAS X extended software package.

DM6B

Capabilities:

Brightfield

  • Differential Interference Contrast
  • Polarizing Imaging

Fluorescence - Metal halide lamp and filter sets:

  • DAPI/UV: Ex 325-375/Em 435-485
  • GFP: Ex 450-490/Em 500-550
  • TRITC: Ex 532-558/Em 570-640
  • TXR: Ex 540-580/Em 592-668
  • Cy5: Ex 590-650/Em 662-738
  • Cy7: Ex 672-747/Em 765-855

Quad Sedat EFW:

  • DAPI/UV: Ex 325-375/Em 430-480
  • FITC: Ex 480-500/Em 507-543
  • TRITC: Ex 542-568/Em 579-631
  • Cy5: Ex 630-660/Em 669-741

Fura:

  • Fura2-ET EFW: Ex 340 and 380/Em 470-550

Fret Set:

  • CFP: Ex 422-432/Em 465-495
  • YFP: Ex 498-510/Em 529-566

Magnification Apertures:

  • Dry: 5x/0.15, 10x/0.30, 20x/0.80, 40x/0.85
  • Water immersion: 20x/0.75, 40x/0.80
  • Oil immersion: 40x/1.25-0.75, 40x/1.30, 63x/1.40-0.60, 63x/1.40, 100x/1.4-0.70, 100x/1.30

Equipped with QE enhanced Orca Flash 4.0 V2 sCMOS camera and LAS X extended software for digital image capture and analysis:

  • Multichannel acquisition and image overlay with labeling
  • Constrained iterative deconvolution in 2-D and 3-D
  • 3-D reconstruction (surface and volume)
  • Time lapse
  • Montage image
  • Analysis, graphs and spreadsheets of data
  • Resolution of camera 2048 x 2048 pixel
  • 100 frames per second (fps) at full resolution in Standard Scan mode or 30 fps in Slow Scan mode

 

LSU